引用本文:	徐冬冬,楼宝,薛宝贵,史会来,詹炜,马世磊,毛国民.黄姑鱼雌核发育的人工诱导及鉴定.海洋与湖沼,2012,43(2):.

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黄姑鱼雌核发育的人工诱导及鉴定
徐冬冬,楼宝,薛宝贵,史会来,詹炜,马世磊,毛国民
浙江省海洋水产研究所,浙江省海洋水产研究所,浙江省海洋水产研究所,浙江省海洋水产研究所,浙江省海洋水产研究所,浙江海洋学院,浙江省海洋水产研究所

摘要:

采用紫外线（UV）灭活的同源精子通过冷休克抑制第二极体排放诱导黄姑鱼的雌核发育。黄姑鱼精子最适的灭活剂量为420 mJ/cm2，紫外照射剂量与黄姑鱼精子和卵子授精后的受精率及孵化率之间表现出明显的Hertwig效应。多次试验筛选的黄姑鱼雌核发育的最适条件为授精后2min在3℃处理8min。雌核发育仔鱼经形态学和流式细胞仪鉴定为二倍体。进一步利用10对微卫星分析了遗传物质在亲本和子代中的传递情况，结果表明雌核发育后代的等位基因完全来自于母本，没有父本基因参与；雌核发育后代在5个位点微卫星位点具有较高的重组率，使其与母本保持了高度同质性。

关键词: 黄姑鱼，雌核发育，人工诱导，紫外灭活，冷休克，微卫星

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附件

ARTIFICIAL INDUCTION OF DIPLOID GYNOGENESIS IN NIBEA ALIBIFLORA AND EVIDENCE FOR FEMALE HOMOGAMETY

Xu Dongdong,Lou Bao,Xue Baogui,Shi Huilai,Zhan Wei,Ma Shilei and Mao Guomin

Marine Fishery Institute of Zhejiang Province,Marine Fishery Institute of Zhejiang Province,Marine Fishery Institute of Zhejiang Province,Marine Fishery Institute of Zhejiang Province,Marine Fishery Institute of Zhejiang Province,Zhejiang Ocean University,Marine Fishery Institute of Zhejiang Province

Abstract:

The induction of diploid gynogenesis in Nibea alibiflora was established using a combination of UV-irradiated homologous sperm and cold shock of the resulting embryo to inhibit the second meiotic division. The optimal UV irradiation of the N. alibiflora sperm for gynogenesis was 420 mJ/cm2. A typical “Hertwig effect” was observed based on the fertilization, hatching and occurrence of haploid by subjecting eggs fertilized with UV-irradiation sperm. Treatment optima for gynogenesis were 3℃ for 8 min at 2min after fertilization based on several experiments. Results obtained from the observations of external morphologies and detection of DNA content in larvae by flow cytometry proved that the larvae were diploid genogenesis. Furthermore, genetic inheritance from the parents to the progeny was examined by 10 microsatellites. The genetic analysis revealed exclusively maternal inheritance in all gynogenetic fry and no paternal contributions were detected. The combination rate was relative high at 5 microsatellite loci in the gynogenetic offspring, and thereby the offspring has high genetic homogeneity to their female parent.

Key words: Nibea albiflora, gynogenesis, artificial induction, UV irradiation, cold shock, microsatellite