引用本文:	隋虎辰,谢国驷,边慧慧,王秀华,张晓华,黄倢.两种多糖作为迟缓爱德华氏菌(Edwardsiella tarda)灭活疫苗佐剂对大菱鲆(Scophthalmus maximus)的免疫保护效果.海洋与湖沼,2012,43(5):1001-1007.

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*两种多糖作为迟缓爱德华氏菌(Edwardsiella tarda)灭活疫苗佐剂对大菱鲆(Scophthalmus maximus)的免疫保护效果*
隋虎辰¹, 谢国驷^2,3, 边慧慧^2,3, 王秀华², 张晓华¹, 黄倢²
1.中国海洋大学海洋生命学院;2.中国水产科学研究院黄海水产研究所海水养殖生物疾病控制与分子病理学实验室;3.上海海洋大学

摘要:

采用黄芪多糖、葡聚糖作为免疫佐剂, 与迟缓爱德华氏菌灭活疫苗配伍后注射免疫大菱鲆, 测定免疫28d后血清中溶菌酶活力、SOD活力、抗体效价和各免疫组的相对保护率(RPS)。结果表明, 添加多糖免疫佐剂能提高疫苗免疫的大菱鲆的各免疫指标, 添加佐剂的免疫组的血清溶菌酶活力、SOD活力和血清效价比单纯的疫苗免疫组显著提高(P<0.05), 2.5mg/ml黄芪多糖混合疫苗免疫组和5mg/ml葡聚糖混合疫苗免疫组的相对保护率最高, 分别达(78.7±1.3)%和(64.0±8.9)%, 且溶菌酶活力、SOD活力及血清效价等指标较其他各组有提高。

关键词: 迟缓爱德华氏菌, 大菱鲆, 黄芪多糖, 葡聚糖, 疫苗, 佐剂

DOI：10.11693/hyhz201205018018

分类号:

基金项目:国家自然科学基金项目, 31172440 号

附件

THE IMMUNE PROTECTION EFFECTS OF TWO POLYSACCHARIDES AS THE ADJUVANTS OF INACTIVATED EDWARDSIELLA TARDA VACCINE IN TURBOT SCOPHTHALMUS MAXIMUS

SUI Hu-Chen¹, XIE Guo-Si^2,3, BIAN Hui-Hui^2,3, WANG Xiu-Hua², ZHANG Xiao-Hua¹, HUANG Jie²

1.College of Marine Life Science, Ocean University of China;2.Laboratory of Maricultural Organism Disease Control and Molecular Pathology, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;3.Shanghai Ocean University

Abstract:

The immune protection effects of two polysaccharides as the adjuvants of inactivated Edwardsiella tarda vaccine in turbot were studied in this research. The vaccines were prepared with two polysaccharides, astragalus polysaccharides (APS) and glucan (Glu) were added to the inactivated E. tarda vaccine separately. Turbots Scophthalmus maximus were inoculated by intraperitoneal (i.p.) injection. The lysozyme activity (Lyz), superoxide dismutase activity (SOD), and antibody titer in the sera were determined after 28 day post-vaccination; meanwhile, the relative percentage survival (RPS) in each group was compared. The results indicated that the addition of polysaccharide adjuvants in the inactivated vaccine could enhance the immune functions to the vaccinated turbots. The Lyz, SOD, and the antibody titer in the sera of the turbots inoculated with the mixtures of inactivated vaccine and each polysaccharide adjuvant were significantly increased (P<0.05). The RPSin the vaccination group with the mixture of inactivated vaccine and 2.5mg/ml APS and the vaccination group with the mixture of inactivated vaccine and 5mg/ml Glu have reached to the best protection effects at (78.7±1.3)% and (64.0±8.9)%, respectively. The SOD, Lyz, and the antibody titer in the best RPSgroups with the two polysaccharides also reached higher levels than those in the other groups did.

Key words: Scophthalmus maximus, Edwardsiella tarda, Astragalus polysaccharides, Glucan, Vaccine, Adjuvant