| 摘要: |
| 利用线粒体16S rRNA基因片段分析北部湾拟穴青蟹群体遗传结构及其扩张历史。结果表明, 16S rRNA 基因片段长度在521—523bp之间, 6 个拟穴青蟹群体具21种单倍型。群体总体水平的单倍型多样性(h)和核甘酸多样性(π)分别为0.6259和0.001851。群体间遗传距离与地理距离之间无相关性。群体间遗传分化系数(Gst)在0.02008—0.04362 之间, 基因流(Nm)值在5.48—12.20 之间。AMOVA分析表明, 群体间遗传变异占0.1%, 群体内遗传变异占99.9%。群体间固定指数(Fst)的绝对值在0.00026—0.02977之间, 暗示群体间没有产生遗传分化。单倍型邻接树和单倍型中介网络图显示, 单倍型没有按采样地形成谱系分支。中性检验和错配分析揭示, 北部湾拟穴青蟹群体在历史上经历过群体扩张。群体扩张时间大致在更新世晚期的0.04698 —0.01879MY前或0.04520—0.01808MY前。 |
| 关键词: 北部湾, 拟穴青蟹, 遗传结构, 群体扩张, 16S rRNA 基因 |
| DOI：10.11693/hyhz201204021021 |
| 分类号: |
| 基金项目:广西科学基金项目, 0991275 号; 广西科学研究与技术开发计划项目, 09322003 号; 广西科学研究与技术开发计划项目, 11107012-11号; 广西海洋生物技术重点实验室主任基金课题, 0801 号 |
附件 |
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| POPULATION GENETIC STRUCTURE AND EXPANSION ANALYSIS OF GREEN MUD CRAB (SCYLLA PARAMAMOSAIN) FROM BEIBU GULF |
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SONG Zhong-Kui1, LI Meng-Yun1,2, NIE Zhen-Ping1, SUN Feng-Yu1,3, ZHAO Peng1, SU Qiong1
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1.Guangxi Key Laboratory for Marine Biotechnology, Guangxi Institute of Oceanology;2.College of Biology and Technology, Hunan Agricultural University;3.Pharmacy and Life Science, South China University
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| Abstract: |
| Mitochondrial 16S rRNA gene fragment was employed to analyze population genetic structure and expansion history of green mud crab (Scylla paramamosain) from Beibu gulf. The 16S rRNA gene fragments ranged from 521 to 523bp in length and 21 haplotypes were defined in six geographic stocks of green mud crab. Total haplotype diversity (h) and nucleotide diversity (π) were 0.6259 and 0.001851, respectively. No correlation was detected between genetic distances and geographic distances. Genetic differentiation coefficients (Gst) of pairwise populations extended from 0.02008 to 0.04362, while gene flows (Nm) from 5.48 to 12.20. An analysis of molecular variance (AMOVA) showed that variance within populations accounted for 0.1%, while among populations accounted for 99.9%. The absolute values of fixation indices in the scope of 0.00026—0.02977 suggested no genetic differentiation for pairwise populations. Neighbour-joining tree and median-joining network generated from haplotype data set displayed no apparent lineage clades association with sampling locations. Neutral test and mismatch-distribution analysis disclosed that the populations of green mud crab from Beibu gulf had ever experienced population expansion, whose expansion time was roughly estimated at the late Pleistocene (0.04698—0.01879MY ago or 0.04520—0.01808MY ago). |
| Key words: Beibu gulf, Scylla paramamosain, Genetic structure, Population expansion, 16S rRNA gene |
