| 摘要: |
| 从东海陆架DH-13站点的表层沉积物中提取环境基因组DNA, 通过PCR和TA克隆构建了细菌和古菌的16S rDNA基因文库, 并对克隆子文库进行系统发育分析。结果表明: 细菌序列以变形菌门(Proteobacteria, 41.5%)居多, 其次是浮霉菌门(Planctomycetes, 10.9%)、放线菌门(Actinobacteria, 8.9%)和CFB group (7.9%), 另外还有少量酸杆菌门(Acidobacteria)、疣微菌门(Verrucomicrobia)、硝化螺旋菌门(Nitrospira)和厚壁菌门(Firmicutes)等; 古菌序列全部来自泉古生菌门(Crenarchaeota), 其中Marine Crenarchaeotic Group I (MG I, 93.6%)是绝对优势菌, 还有少量序列属于Miscellaneous Crenarchaeotic Group (MCG)、Marine Benthic Group C (MBGC)和Marine Benthic Group A (MBGA)。文库多样性分析结果显示东海陆架表层沉积物中有着丰富多样的微生物群落, 细菌的多样性更为显著。 |
| 关键词: 表层沉积物, 微生物多样性, 16S rDNA |
| DOI：10.11693/hyhz201204018018 |
| 分类号: |
| 基金项目:浙江省自然科学基金项目, LY12C03003 号; 浙江省科技厅公益性技术应用研究项目, 2011C31017 号; 舟山市科技局海洋类项目, 10248 号; 浙江省大学生科技创新项目, 2010R411002 号 |
附件 |
|
| A PRELIMINARY STUDY OF MICROBIAL DIVERSITY OF THE SURFACE LAYER SEDIMENTS FROM THE EAST CHINA SEA |
|
WANG Jian-Xin, XU Xian-En, ZHOU Lian-Lian, TAO Shi, LIU Xue-Zhu, SHI Ge
|
|
Zhejiang Ocean University, Laboratory for Marine Living Resources and Molecular Engineering
|
| Abstract: |
| The environmental total DNAs were directly extracted from the surface layer sediment of the East China sea. The bacterial and archaeal 16S rDNA gene libraries were generated by using PCR and TA cloning. Phylogenetic results show that there are eight phyla in the bacteria domain: Proteobacteria (41.5%), Planctomycete (10.9%), Actinobacteria (8.9%), CFB group (7.9%), Acidobacteria, Verrucomicrobia, Nitrospira and Firmicutes, the phylum Proteobacteria is predominant. In the archaea domain, all clones were belonged to Crenarchaeota and no one Euryarchaeota clone was detected. There are four divisions in the Crenarchaeota kingdom: MarineCrenarchaeotic Group I (MG I, 93.6%), Miscellaneous Crenarchaeotic Group (MCG), Marine Benthic Group C (MBGC), Marine Benthic Group A (MBGA), and MG I is the dominating group. DOTUR statistics of 16S rDNA gene sequences from the libraries indicating a high richness in the sediment microbial communities. |
| Key words: Surface layer sediment, Microbial diversity, 16S rDNA |
