引用本文:	衣启麟,于海洋,王兴莲,王志刚,王旭波,齐洁,张全启.静水压力诱导牙鲆( Paralichthys olivaceus)四倍体的条件优化.海洋与湖沼,2012,43(2):382-388.

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*静水压力诱导牙鲆( Paralichthys olivaceus)四倍体的条件优化*
衣启麟,于海洋,王兴莲,王志刚,王旭波,齐洁,张全启
中国海洋大学海洋生命学院海洋生物遗传育种教育部重点实验室

摘要:

采用静水压力抑制受精卵第一次卵裂的方法, 进行了诱导牙鲆四倍体的研究。结果表明, 将受精卵保持在(15.5±0.5) ℃, 从受精后70min 开始用55MPa 的压力处理6min后, 正常仔鱼孵化率最高达到15.6%, 此时的四倍体诱导率也最高达到63.3% 。利用获得的最佳诱导条件处理获得了数千尾体长8 —15cm 的幼鱼, 流式细胞仪检测和红血球长径测量结果显示, 处理组幼鱼中四倍体约占13.3%, 说明四倍体培育成功。该成果为开展牙鲆多倍体育种奠定了基础。

关键词: 牙鲆, 四倍体, 静水压力, 多倍体育种

DOI：10.11693/hyhz201202029029

分类号:

基金项目:山东省良种工程重大课题“优质抗病速生鱼类良种选育”, 2008 —2014; 国家863 计划课题“海洋动物细胞分子育种关键技术”, SS2012AA100802 号; 山东省海洋生物学泰山学者岗位经费资助, ts2010021 号

附件

PRODUCTION OF VIABLE TETRAPLOID OLIVE FLOUNDER (PARALICHTHYS OLIVACEUS) BY HYDROSTATIC PRESSURE SHOCK

YI Qi-Lin^1,2, YU Hai-Yang^1,2, WANG Xing-Lian^1,2, WANG Zhi-Gang^1,2, WANG Xu-Bo^1,2, QI Jie^1,2, ZHANG Quan-Qi^1,2

1.College of Marine Life Science, Ocean University of China;2.Key Laboratory of Marine Genetics and Breeding, Ministry of Education

Abstract:

Viable tetraploid olive flounder (Paralichthys olivaceus) were hatched under hydrostatic pressure shock. The result showed that optimal hatching rate for tetraploid individuals was achieved by 6 min of pressure shock (55Mpa) at (15.5±0.5) ℃, 70min after fertilization. Under these conditions, the hatching rate of the treated eggs was 15.6%. Chromo-some observation and counting of maximum number of nucleolus showed that the tetraploid rate was 63.3%. Several thousands of young fish with body length of 8—15cm were obtained using this method. Both flow-cytometry and erythro-cyte diameter proved that 13.3% of the young fish were tetraploids. The successful production of tetraploid individuals provided possibilities for future polyploid breeding using this fish species.

Key words: Paralichthys olivaceus, Tetraploid, Hydrostatic pressure shock, Polyploid breeding