引用本文:	武玉学,靳挺,王建平,乐韵,林桂芳.天然海藻絮凝剂海藻酸钠回收带鱼(Trichiurus haumela)鱼糜漂洗液中蛋白质的研究.海洋与湖沼,2012,43(2):335-339.

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*天然海藻絮凝剂海藻酸钠回收带鱼(Trichiurus haumela)鱼糜漂洗液中蛋白质的研究*
武玉学¹, 靳挺¹, 王建平², 乐韵³, 林桂芳²
1.浙江大学宁波理工学院生物与化学工程分院;2.宁波市海洋与渔业研究院;3.宁波市海洋与渔业执法支队

摘要:

采用海藻酸钠絮凝剂法、双缩脲法、重铬酸钾回流氧化等方法, 研究了回收带鱼鱼糜漂洗液中蛋白质的最佳条件, 以期为鱼糜漂洗液蛋白质的利用提供参考。结果表明, 海藻酸钠回收带鱼鱼糜漂洗液蛋白质的最佳条件为: 海藻酸钠添加量为1.11mg/ml, 鱼糜漂洗液蛋白浓度16.54mg/ml, pH 4.6, 温度4 ℃, 处理时间20min; 在此条件下蛋白质回收率达86.68%, COD去除率达58.67%。实验表明海藻酸钠对鱼糜漂洗液蛋白质去除效果非常显著。

关键词: 带鱼, 鱼糜漂洗液, 海藻酸钠, 蛋白质, 回收

DOI：10.11693/hyhz201202022022

分类号:

基金项目:宁波市农业科技创新创业重点项目, 2011C92019 号; 国家重点星火计划项目, 2010GA701005 号

附件

RESEARCH ON THE RECOVERY OF PROTEIN FROM SURIMI WASH WATER OF HAIRTAIL TRICHIURUS HAUMELA BY NATURAL ALGAE FLOCCULANT SODIUM ALGINATE

WU Yu-Xue¹, JIN Ting¹, WANG Jian-Ping², LE Yun³, LIN Gui-Fang²

1.School of Biotechnology and Chemical Engineering, Ningbo Institute of Technology , Zhejiang University;2.Ningbo Ocean & Fishery Institute;3.Ningbo Ocean and Fishery Enforcement Detachment

Abstract:

To obtain the optimum conditions of sodium alginate flocculants used for the recovery of protein from surimi wash water of hairtail Trichiurus haumela, the methods of sodium alginate flocculants, biuret and potassium dichromate oxidation were adopted. Results showed that the optimum recovery conditions of sodium alginate were as follows: sodium alginate 1.11mg/ml; protein concentration of surimi wash water 16.54mg/ml; pH 4.6; temperature 4℃; time 20min. Under the above condition, protein recovery ratio and removal ratio of COD was 86.68% and 58.67%, respectively. The removal effect of protein from surimi wash water was obvious by adding sodium alginate.

Key words: Hairtail Trichiurus haumela, Surimi wash water, Sodium alginate, Protein, Recovery