引用本文:	邓云翠,王辉,李艳红,朱晓闻,罗明明,王亚男.Cd²⁺与Zn²⁺的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响.海洋与湖沼,2012,43(2):323-328.

【打印本页】【HTML】【下载PDF全文】【查看/发表评论】【下载PDF阅读器】【关闭】

过刊浏览高级检索

本文已被：浏览 2703次下载 2607次	码上扫一扫！
分享到：微信更多字体:加大+\|默认\|缩小-
*Cd²⁺与Zn²⁺的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响*
邓云翠, 王辉, 李艳红, 朱晓闻, 罗明明, 王亚男
广东海洋大学水产学院

摘要:

采用中心组合设计和响应曲面法在实验室条件下研究了Cd²⁺与Zn²⁺对马氏珠母贝受精率的影响。Cd²⁺、Zn²⁺的浓度范围分别为0.030—20mg/L、0.100—20mg/L。结果表明: Cd²⁺与Zn²⁺两因子对马氏珠母贝受精率的一次效应极显著( P <0.01); Cd²⁺与Zn²⁺两因子一次互作效应极显著( P <0.01) 。经响应曲面法分析, 随着Cd²⁺与Zn²⁺浓度的增加受精率呈下降趋势, 当Zn²⁺浓度在最低浓度(0.100mg/L)时, 受精率随着Cd²⁺浓度的上升而下降; 当Cd²⁺浓度在最低浓度(0.030mg/L)时, 受精率随Zn²⁺浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd²⁺、Zn²⁺间关系的模型方程( R² = 0.996, Adj.R² = 0.987, Pred.R² = 0.887, P <0.01) 并可用于预测Cd²⁺、Zn²⁺对马氏珠母贝受精率的影响。

关键词: 马氏珠母贝, 受精率, Cd²⁺, Zn²⁺

DOI：10.11693/hyhz201202020020

分类号:

基金项目:广东省教育厅工程中心建设项目, GCZX-A0909 号; 广东省科技厅科技计划重大专项, 2010B020201014 号; 科技部国家星火计划项目, 2011GA780001 号

附件

COMBINED EFFECTS OF Cd²⁺ AND Zn²⁺ ON THE FERTILIZATION RATE IN PINCTADA MARTENSII (DUNKER)

DENG Yun-Cui, WANG Hui, LI Yan-Hong, ZHU Xiao-Wen, LUO Ming-Ming, WANG Ya-Nan

Fisheries College of Guangdong Ocean University

Abstract:

Using the central composite face-centered design (CCF) and response surface methodology, the combined effects of Cd²⁺ (0.030—20.000mg/L) and Zn²⁺ (0.100—20.000mg/L) on the fertilization rate in Pinctada martensii were studied under laboratory conditions. The results showed that the effects of two factors Cd²⁺ and Zn²⁺ on the fertilization rate in P. m a r t e n s i i were highly significant ( P <0.01), and effect of Cd²⁺ was more significant than that of Zn²⁺. The inter-action between the two factors was also highly significant (P <0.01). Based on the response surface plots, it was found that with the increased concentration of Cd²⁺ and Zn²⁺ , the fertilization rate was decreased. When Zn²⁺ concentration was at its lowest level (0.100mg/L), the fertilization rate decreased with the increased Cd²⁺ concentration. When Cd²⁺ concentration was at its lowest level (0.030mg/L), the fertilization rate was first decreased and then increased as the concentration of Zn²⁺ increased. The model equation of the fertilization rate towards Cd²⁺ and Zn²⁺ was established, with R² = 0.996, Adj.R² = 0.987 and Pred. R² = 0.887 (P <0.01). The model equation could be practically used for forecasting the effects of Cd²⁺ and Zn²⁺ on the fertilization rate in P. martensii.

Key words: Pinctada martensii (Dunker), Fertilization rate, Cd²⁺, Zn²⁺