| 摘要: |
| 应用光学和电子显微成像技术对黄颡鱼鳃的表面形态特征、鳃小片血管分布及其内部超微结构进行了观察。结果表明, 黄颡鱼鳃耙呈长锥形, 鳃弓表面具棘状小刺, 鳃小片呈褶皱状, 镶嵌排列在长条形鳃丝两侧。光镜可观察到鳃小片两侧红色的入鳃和出鳃动脉以及中部片状毛细血管网。鳃耙、鳃弓、鳃丝及鳃小片表面均被扁平上皮细胞覆盖, 其表面微嵴形态结构各异。鳃弓和鳃丝表面有线粒体密集细胞和粘液细胞开口, 鳃小片表面未发现分泌细胞开口但附着大量粘液。鳃小片主要由单层或数层上皮细胞和由支持细胞包裹的血管腔构成。线粒体密集细胞分布于鳃小片边缘和血管周围, 可分为两个亚型。黄颡鱼鳃上皮细胞和线粒体密集细胞的超微结构特征与其相应的呼吸功能和体内离子转运、能量代谢生理功能相适应。 |
| 关键词: 黄颡鱼, 鳃, 扁平上皮细胞, 线粒体密集细胞, 超微结构 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金重点基金项目, U0936602 号; 福建省教育厅科技项目, JB09233 号; 宁德师范学院“服务海西建设”资助项目, 2010H307; 宁德师范学院教授培养工程资助项目 |
附件 |
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| FINE STRUCTURE OF GILLS IN TELEOST (PELTEOBAGRUS FULVIDRACO) |
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LUO Fen1, CHEN Li-Qiang2, KANG Bin2
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1.Department of Bioengineering, Ningde Teacher College;2.Asian International Rivers Center, Yunnan Key Lab of International Rivers and Transboundary Eco-security, Yunnan University
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| Abstract: |
| Using optical and electron microscopic imaging techniques, we studied the fine structure of gills in teleost (Pelteobagrus fulvidraco), including surface morphology, lamellar vascular distribution, and internal ultrastructure of gill filament. The results showed that the prick-shaped gill rakers were presented on the anteromedial side of the arch opposite to the gill filaments, and abundant small spines were presented on the dorsal surface of the gill arch. Many gill lamellae were arranged on both sides of each filament, and interwove with each other. The red afferent filamental artery, the efferent filamental artery, and the capillary network of lamellae were clearly observed under an optical microscope. The gill rakers, gill arches, and branchial filaments were all covered by a layer of stratified epithelium, whereas distinct morphology of microridges were presented on the surface of pavement cells. In the stratified epithelium, the pillar cells, the mucous cells, the pavement cells, and the mitochondria-rich cells were identified, and the ultrastructure of all these cell types was described. Two kinds of holes, which were considered to be the opening of the mucous cells or the mitochondria-rich cells, were located in the circular ridge of the gill arches and gill filaments, showing clear distinction from each other. On the contrary, no hole was found on the surface of lamellae, which was covered by a mucus layer. The basic structure of the secondary lamellae consisted of one or two outer epithelial layers and intermediate blood channels, which were coated by the pillar cells and their flanges. The mitochondria-rich cells that lined the lamellae or around the vascular included two cell types, i.e., the light cells and the dark cells. The relationships between the ultrastructure and function of the pavement and mitochondria-rich cells were also discussed. |
| Key words: Pelteobagrus fulvidraco, Gill, Pavement cells, Mitochondria-rich cells, Ultrastructure |
