引用本文:	苗亮,王天柱,李明云,汤先念,王曙,安钦,徐万土.同源和异源精子诱导大黄鱼(Pseudosciaena crocea)雌核发育的胚胎发育比较及子代 SSR 遗传标记分析.海洋与湖沼,2011,42(3):419-424.

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*同源和异源精子诱导大黄鱼(Pseudosciaena crocea)雌核发育的胚胎发育比较及子代 SSR 遗传标记分析*
苗亮¹, 王天柱¹, 李明云¹, 汤先念¹, 王曙¹, 安钦¹, 徐万土²
1.宁波大学教育部应用海洋生物技术重点实验室;2.象山港湾水产苗种有限公司

摘要:

以紫外灭活的同源(大黄鱼)精子和未灭活的异源(鮸鱼)精子为激活源, 采用冷休克处理的方法诱导了大黄鱼雌核发育二倍体, 进行胚胎发育和 SSR 标记分析的比较研究。结果表明, 异源组的受精率和孵化率高于同源组, 但存活率低于同源组; 两组中经冷休克处理未能恢复倍性的胚胎发育畸形而陆续死亡, 恢复倍性的胚胎在发育程序上均与普通大黄鱼相同, 但各阶段出现时间较对照组滞后; SSR 分析显示同源组子代中有 16.7%出现父本条带, 异源组子代均未出现父本条带。以灭活的同源(大黄鱼)精子和未经灭活的

关键词: 大黄鱼, 鮸鱼, 雌核发育, 胚胎发育, SSR 标记

DOI：10.11693/hyhz201103013013

分类号:

基金项目:国家“863”高技术研究发展计划, 2006AA10A405 号; 浙江省科技厅重大专项, 2009C12077 号; 宁波市农业科技示范园区项目, 2010YQ22 号。

附件

COMPARISON OF EMBRYONIC DEVELOPMENT AND SSR ANALYSIS OF GYNOGENESIS IN PSEUDOSCIAENA CROCEA INDUCED BY HOMO- AND HETEROLOGOUS SPERM

MIAO Liang¹, WANG Tian-Zhu¹, LI Ming-Yun¹, TANG Xian-Nian¹, WANG Shu¹, AN Qin¹, XU Wan-Tu²

1.Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University;2.Gangwan Aquacultural Seeding Co. Ltd. of Xiangshan County

Abstract:

Gynogenesis was induced in Pseudosciaena crocea with heterologous (Miichthys miiuy) sperm and UV-irradiated homologous sperm, and the embryonic development and SSR patterns were compared. The fertilization and hatching rates of hetero-gynogenesis were higher than homo-gynogenesis, although the survival rate was lower. In the two gynogenesis groups, the embryos which failed to inhibit the extrusion of polar body showed obvious haploid syndrome, the embryos which were successful diploidized had the same process with the normal diploid embryos. However, the developing speed of gynogenetic diploid was slower than the control group. The delay may be due to the disturbance to the cell division cycle caused by cold shock. In homo-gynogenesis, SSR analysis at loci KPC9 and KPC45 showed in 24 tested offsprings, with four individuals showing the paternal bands, suggesting that there were genetic leakage of the paternal fish. In hetero-gynogenesis, SSR analysis at locus KPC43 showed 18 tested offsprings with all individuals showing the maternal specific band only, indicating that the heterologous sperm can avoid paternal gene inflowing to the offspring. In the gynogenesis induction studies, the selection of homo- or heterologous sperm should be based on their relative merits.

Key words: Pseudosciaena crocea, Miichthys miiuy, Gynogenesis, Embryo development, SSR markers