| 摘要: |
| 采用气相色谱检测的方法, 进行大西洋庸鲽精液脂肪酸组成分析及激素 GnRHa 诱导对其组成影响的研究。结果表明, 大西洋庸鲽精液中含量最高的脂肪酸种类为 22:6n-3 (DHA, 二十二碳六烯酸), 占总脂肪酸比例 25.67%±0.94%; 其次为 16:0(PA, 棕榈酸)、20:5n-3 (EPA, 二十碳五烯酸); 重要必需不饱和脂肪酸 20:4n-6 (AA, 花生四烯酸)含量较低, 为 1.76%±0.01%。精液中高不饱和脂肪酸(PUFA)含量较高, 为 44.25%±0.30%; 饱和脂肪酸(SAT)含量为 27.72%±0.22%。重要脂肪酸比例DHA/EPA 为 2.33±0.26; EPA/AA 为 6.30±0.51; n-3/n-6 为 9.22±0.60。激素诱导未对精液中脂肪酸组成产生显著影响。重要必需脂肪酸 DHA、EPA、AA, 以及 n-3、n-6 等重要种类的脂肪酸总量在激素诱导组与非诱导对照组样品间无显著差异; 在激素诱导后的三个取样时间的样品间也无显著差异(P<0.05)。
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| 关键词: 大西洋庸鲽, 精液, 脂肪酸, 激素 |
| DOI：10.11693/hyhz201006009009 |
| 分类号: |
| 基金项目:国家鲆鲽类产业技术体系建设专项资金, nycy-tx-50 号; 国家自然科学基金资助项目, 31072212 号, 41076100 号 |
附件 |
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| CHARACTERIZATION OF FATTY ACIDS COMPOSITION IN THE MILT OF ATLANTIC HALIBUT (HIPPOGLOSSUS HIPOGLOSSUS L.) AND ITS CHANGES DUE TO GnRHa IMPLANTATION |
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DING Fu-Hong1, Santosh P. Lall2, LI Jun3, LEI Ji-Lin1, MA Ai-Jun1
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1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.National Research Council Canada, Institute for Marine Biosciences;3.Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| In the present study, fatty acid analyzation of halibut milt was conducted by gas chromatography to provide its physiological characterization. The effect of hormone implantation on milt fatty acid composition of Atlantic halibut was also investigated. The results indicated that the majority of fatty acids in Atlantic halibut (Hippoglossus hipoglossus L.) milt was 22:6n-3 (docosahexanoic acid, DHA) with concentration of 25.67%±0.94%, followed by 16:0 (palmitic acid) and 20:5n-3 (eicosapentanoic acid, EPA) with concentration of 17.39%±0.23%, and 11%±0.85%, respectively. The results also showed 20:4n-6 (archidonic acid, AA) with concentration as low as 1.76%±0.01%. The proportion of total saturated fatty acids (SAT) was 27.72%±0.22%, with polyunsaturated fatty acids (PUFA) of 44.25%±0.30% and monounsaturaed fatty acids (MUFA) of 26.85%±0.51%. The ratio of DHA/EPA and EPA/AA were 2.33±0.26 and 6.30±0.51, respectively. And n-3/n-6 was found to be 9.22±0.60. The analyzation of GnRHa implanted milt indicated that the essential fatty acids (DHA, EPA, and AA) and the ratios between important fatty acid series (n-3, n-6, PUFA, and SAT) were not affected by the hormone implantation (P<0.05).
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| Key words: Atlantic halibut Hippoglossus hipoglossus, Milt, Fatty acids, Hormone |
