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| 环境因子对南极菌 Pseudoalteromonas sp.S-15-13多糖合成关键酶 UGD 基因表达的影响 |
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李 江1,2, 沈继红1,2, 何培青1,2, 林学政1,2, 谭姣姣3, 李光友1,2
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1.国家海洋局海洋生物活性物质重点实验室;2.国家海洋局第一海洋研究所;3.青岛科技大学化工学院
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| 摘要: |
| 以南极菌 Pseudoalteromonas sp.S-15-13 为材料, 采用实时定量 PCR 的方法研究了温度、冻融循环及培养基中 NaCl、葡萄糖含量和 pH 对多糖合成关键酶基因 ugd 表达水平的影响。结果表明, 低温有利于ugd的表达, 在2℃和10℃培养温度下, 培养24 h后ugd的表达量约为20℃时的4倍; 冻融循环后, ugd 的表达量上调, 第 2 个冻融循环后 ugd 的表达量较对照提高了 6.85 倍; NaCl 对 ugd的影响表现为低促高抑, 即 NaCl 含量为 6.0%时 ugd 的表达量是对照(3.0%)的 3.97 倍, 但含量达 9.0%时ugd表达量仅为对照的2/5; 葡萄糖能够提高ugd的表达量, 当含量为2.0%时ugd表达量为对照的13.68 倍; 在一定范围内(pH 5.0—8.0), pH 的改变会促进ugd 的表达, 当 pH 为 6.0 时 ugd 表达量约为pH 7.0 时的 2.15 倍。
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| 关键词: 南极菌 Pseudoalteromonas sp.S-15-13, Real-time PCR, UGD, 环境因子 |
| DOI：10.11693/hyhz201006004004 |
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| 基金项目:国家自然科学基金, 40706053 号; 国家 863 科技计划重点项目, 2007AA091905 号 |
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| EFFECTS OF ENVIRONMENTAL FACTORS ON THE UGD GENE EXPRESSION OF ANTARCTIC BACTERIUM PSEUDOALTEROMONAS SP. S-15-13 |
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LI Jiang1,2, SHEN Ji-Hong1,2, HE Pei-Qing1,2, LIN Xue-Zheng1,2, TAN Jiao-Jiao3, LI Guang-You1,2
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1.Key Laboratory of Marine Bio-active Substances, State Oceanic Administration;2.First Institute of Oceanorgarphy, State Oceanic Administration;3.College of Chemical Engineering, Qingdao University of Science & Technology
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| Abstract: |
| As a key enzyme, UGD plays important roles in synthesis of polysaccharides, on which this paper studied. In this paper, effects of environmental factors(such as: temperature, freeze-thaw, NaCl, glucose and pH) on ugd gene expression was studied with real-time PCR for Pseudoalteromonas sp.S-15-13 isolated from sea ice (62°00?S, 68o30?E)samples collected by the 19th Antarctic Scientific Research Expedition of China during 2002—2003. It was showed that the ugd expression level of S-15-13 grown at 2℃ and 10℃was about 4 times compared to that of grown at 20℃ after 24h culture; the expression level of ugd was 6.85 times higher than the untreated sample after two freeze-thaw cycle of S-15-13; 6.0% NaCl could increase the expression level of ugd nearly three times compare to that of 3.0%, but when the NaCl concentration up to 9.0% the expression level of ugd was only 2/5 of control; the expression level of ugd was improved 12.68 times when the glucose concentration added to 2.0% compared to that of control; pH change (5.0—8.0)will promote the expression of ugd, 2.15 times expression was enhanced when pH down to 6.0 from 7.0. This study on ugd may deepen our understanding of the adaptation mechanism of Antarctic bacteria living in harsh environment.
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| Key words: Antarctic bacterium Pseudoaltermonas sp. S-15-13, Real-time PCR, UGD, Environmental factors |