| 摘要: |
| 采用3H-TdR 同位素示踪法研究了病原性溶藻弧菌和9 株拮抗菌对大黄鱼表皮粘液的粘附动力学。结果表明, 10 株细菌的粘附作用都符合饱和粘附动力学特征; R4、R13 和R32 等3 株拮抗菌的最大粘附量高于溶藻弧菌; 9 株拮抗菌的分离常数都高于溶藻弧菌; R32 的亲和指数大于溶藻弧菌,其余8 株拮抗菌的亲和指数都小于溶藻弧菌。测定了9 株拮抗菌对病原性溶藻弧菌粘附大黄鱼表皮粘液的竞争、排斥和置换作用, 结果显示：9 株拮抗菌在竞争条件下都能极显著减少溶藻弧菌对表皮粘液粘附(P<0.01); 在粘附排斥方面, R4、R13、J26、J28、J312、Y59 等6 株拮抗菌能显著排斥病原性溶藻弧菌对大黄鱼表皮粘液的粘附作用(P<0.05); 在粘附置换方面, J26、J28 等2 株拮抗菌能极显著(P<0.01)降低病原性溶藻弧菌对大黄鱼表皮粘液的粘附量。研究结果表明, 病原性溶藻弧菌对大黄鱼表皮粘液有较强的粘附作用; 9 株拮抗菌能够抑制溶藻弧菌对大黄鱼表皮粘液的粘附作用, 其中竞争作用效果最好, 排斥作用次之, 置换作用的效果最差; 拮抗菌对病原菌粘附的抑制效果受多种因素的影响, 各菌株的粘附动力学参数有重要的影响作用。
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| 关键词: 大黄鱼, 溶藻弧菌, 拮抗菌, 表皮粘液, 粘附 |
| DOI：10.11693/hyhz200906011011 |
| 分类号: |
| 基金项目:国家863 计划项目, 2007AA09Z115 号; 厦门市科技项目, 3502Z20073019 号 |
附件 |
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| INFLUENCE OF ANTAGONISTIC BACTERIA ON ADHESION OF PATHOGENIC VIBRIO ALGINOLYTICUS TO SKIN MUCUS OF PSEUDOSCAENA CROCEA |
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GUO Guo-Jun1,2, YAN Qing-Pi1, ZOU Wen-Zheng1, ZHOU Ren-Sun1, CHEN Chang-Sheng1, CHEN Qiang3
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1.Fisheries College of Jimei University;2.Zhengzhou College of Animal Husbandry Engineering;3.Fisheries College, Ocean University of China
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| Abstract: |
| The adhesion kinetics of pathogenic Vibrio alginolyticus and 9 strains of antagonistic bacteria to skin mucus of Pseudosciaena crocea were studied by using of 3H-TdR as isotope tracer. The results show that the adhesion of the 10 strains complies with saturation kinetics; the maximum number of adhered bacterial cells (em) is greater in strains R4, R13 and R32 than V. alginolyticus; the dissociation constant (kx) of all the 9 strains of antagonistic bacteria is higher than that of V. alginolyticus; the affinity index of strain R32 is higher but lower in the rest 8 strains than that of V. alginolyticus. The conditions of competition, exclusion and displacement of the bacterial adhesion to the skin mucus by the 9 strains of antagonistic bacteria were also investigated. The results show that all of the 9 strains of antagonistic bacteria can remarkably reduce the adhesion V. alginolyticus to some extents under conditions of competition, exclusion, and displacement. Pathogenic V. alginolyticus can strongly adhere to the skin mucus of P. crocea; the 9 strains of antagonistic bacteria can inhibit the bacterial adhesion with the best result under competition condition. Inhibition to the bacterial adhesion depends on many factors of which bacterial adhesive kinetics is the most important one.
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| Key words: Pseudosciaena crocea, Vibrio alginolyticus, Antagonistic bacteria, Skin mucus, Adhesion |
