| 摘要: |
| 对笛鲷属9 种鱼的线粒体DNA 16S rRNA 基因片段进行了PCR 扩增, 扩增产物经纯化后测序, 得到了长度为561bp 的分析序列。结合GenBank 中的另外9 种笛鲷的16S rRNA 同源序列计算得出A、T、G、C 的含量平均为28.5%、22.1%、23.6%和25.8%, AT 含量稍高于GC 含量, 18 种笛鲷碱基组成差异不大。利用MEGA3.1 软件对所得序列进行比对后检测到72 个变异位点, 其中包括简约信息位点44 个, 在变异位点中75.61%的碱基替换是由于发生了转换, 转
|
| 关键词: 笛鲷属, 16S rRNA, 序列比较, 分子系统学 |
| DOI：10.11693/hyhz200905007007 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 30671610 号; 国家科技支撑计划项目, 2007BAD29B03 号; 广东省科技计划项目重大专项,A3050202 号 |
附件 |
|
| SEQUENCE COMPARISON AND SYSTEMATIC ANALYSIS OF mtDNA 16S rRNA GENOMIC FRAGMENTS OF LUTJANUS |
|
LIU Chu-Wu, XU Tian-Jun, LIU Li, GUO Yu-Song, DONG Qiu-Fen
|
|
Fisheries College, Guangdong Ocean University
|
| Abstract: |
| MtDNA 16S rRNA genomic fragments of nine Lutjanus were amplified by PCR; and the purified product yielded a systematic sequence with 561bp being sequenced. The contents of A, T, G and C are respectively 28.5%, 22.1%, 23.6% and 25.8%. The contents of AT are slightly higher than those of GC, and the difference in base composition in 18 Lutjanus species is the minimum. Comparison with MEGA 3.1 software revealed 72 variation loci, of which 44 are information loci. For base replacement, 75.61% are caused by transition in it, and the ratio of transition/transversion is 3.1. The results of heredity distances computing show that the sequence difference is from 0.0193 to 0.0680, of which those of L. russelli and L. fulviflamma, L. russelli and L. vaigiensis are the smallest, while those of L. erythropterus and L. fulviflamma, L. erythropterus and L. vitta are the largest. By choosing Argyrops spinifer as outgroup and integrating the 16S rRNA homologous sequences of other nine Lutjanus from GenBank, the molecular phylogenetic trees with NJ method was constructed. The result shows that the nine Lutjanus in the research is away from the other nine’s in terms of evolutionary relationship, and the nine Lutjanus from South China Sea keep relative long heredity distance between each other with obvious genetic diversity.
|
| Key words: Lutjanus, 16S rRNA, Sequence comparison, Molecular systematic analysis |
