引用本文:	陈桃英,柳俊秀,李水军,刘利平,何培民.膝沟藻毒素液质联用快速检测及其在海洋生物体内的累积.海洋与湖沼,2009,40(1):88-93.

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膝沟藻毒素液质联用快速检测及其在海洋生物体内的累积
陈桃英¹, 柳俊秀¹, 李水军², 刘利平¹, 何培民¹
1.上海海洋大学水产与生命学院;2.上海市徐汇区中心医院中心实验室

摘要:

采用高效液相色谱-质谱联用技术分析了微小亚历山大藻中的膝沟藻毒素(Gonyautoxin, GTX_1,4 和GTX_2,3)以及蒙古裸腹溞和菲律宾蛤仔摄食该藻后对膝沟藻毒素的累积状况。结果表明, 采用高效液相色谱-质谱联用方法, 可快速(4h 内)检测出这三种海洋生物中的膝沟藻毒素, 且培养的微小亚历山大藻每个细胞的膝沟藻毒素含量为9.46fmol。蒙古裸腹溞摄食微小亚历山大藻2h、18h、24h 后, 其体内GTX1,4 的含量分别为4.8908、12.6241、31.4968mg/g,GTX_2,3的含量分别为1.656、3.8533、10.9026 mg/g, 且对GTX_1,4 积累量比GTX_2,3高, 在24h 内GTX_1,4 含量是GTX_2,3 的2.89 倍。菲律宾蛤仔摄食微小亚历山大藻2h、24h 和48h 后, 肌肉中GTX_1,4 含量分别为3.1271、3.6709 和4.6906 μg/g, GTX_2,3 含量分别为7.1874、8.1675 和9.1476 μg/g; 内脏团中GTX_1,4 含量分别为4.7088、4.2178 和 5.5064 μg/g, GTX_2,3 含量分别为7.6774、9.1476 和13.066 μg/g, 表明GTX_1,4 和GTX_2,3 在肌肉中含量逐步增加, 且GTX_2,3 累积量比GTX_1,4 高, 在48h 内肌肉GTX_2,3 含量是GTX_1,4 的1.95 倍, 内脏团GTX_2,3 含量是GTX_1,4 的2.37 倍。在停食净水培养96h 后, 内脏团和肌肉对GTX_1,4 消解率已达100%, 而肌肉和内脏团对GTX_2,3 消解率分别为16.1%和45%。本文结果表明采用高效液相色谱-质谱联用方法能快速测定赤潮藻、浮游动物和贝类中的膝沟藻毒素含量, 但不同生物对膝沟藻毒素有不同的累积方式。

关键词: 高效液相色谱-质谱联用, 微小亚历山大藻, 蒙古裸腹溞, 菲律宾蛤仔, 膝沟藻毒素, 累积

DOI：10.11693/hyhz200901015015

分类号:

基金项目:上海市优秀学科带头人计划项目, 08XD14037 号; 上海市青年科技启明星计划项目, 08QA1405900 号; 上海市登山计划项目, 06dz12015 号; 上海市教委重点科研项目, 科04-131 号; 上海市重点学科建设项目, Y1101 号; 上海市教委重点学科项目,J50701 号; 上海市优秀青年教师选拔基金项目, 科07-10 号; 上海海洋大学博士启动基金项目, 科06-310 号。

附件

GONYAUTOXIN: HPLC-MS DETECTION AND ACCUMULATION IN MARINE ORGANISMS

CHEN Tao-Ying¹, LIU Jun-Xiu¹, LI Shui-Jun², LIU Li-Ping¹, HE Pei-Min¹

1.College of Fisheries and Life Science, Shanghai Ocean University;2.Central Laboratory, Shanghai Xuhui Central Hospital

Abstract:

The gonyautoxins (GTX, including GTX_1,4 and GTX_2,3) in Alexandrium minutum, Moina mongolica Daday, and Ruditapes philippinarum, which fed with A. minutum, were analyzed by HPLC-MS (High Performance Liquid Chromatography coupled with Mass Spectrometry). The results showed that the GTX content in these species could be detected with HPLC-MS. The GTX content per cell in A. minutum was 9.46fmol. In M. mongolica fed with A. minutum for 2h, 18h and 24h, GTX_1,4 content was 4.8908, 12.6241, 31.4968mg/g, and GTX_2,3 content at 1.656, 3.8533, 10.9026 mg/g, respectively, which indicates that GTX content in M. mongolica increased with the exposure time to A. minutum, and GTX_2,3 was 2.89 times of GTX_1,4 content. In R. philippinarum fed with A. minutum for 2h, 24h, and 48h, the GTX_1,4 content in muscles was 3.1271, 3.6709, and 4.6906 μg/g, GTX_2,3 content at 7.1874, 8.1675 and 9.1476 μg/g, and GTX_1,4 content in viscera was 4.7088, 4.2178 and 5.5064 μg/g, respectively, indicating that the GTX content in R. philippinarum increased with the exposure time to A. minutum, and GTX_2,3 was accumulated more than GTX_1,4; and GTX content in viscera was greater than that in mussel: the GTX_2,3 content in muscles in 48h was 1.95 times of that GTX_1,4, and the GTX_2,3 content in viscera was 2.37 times of that GTX_1,4. After animals were starved for 96h, the GTX_1,4 content in muscles and viscera reduced to 0, and GTX_2,3 content reduced by 16.1% and 45%, respectively. The results indicate that HPLC-MS can be used to detect rapidly the GTX in alga, zooplankton, and shellfish. The accumulation of GTX was different in higher trophic-level organisms.

Key words: HPLC-MS, Alexandrium minutum, Moina mongolica, Ruditapes philippinarum, Gonyautoxin, Accumulation