引用本文:	汝少国,姜明,李永祺,马英杰.中国对虾育苗期球形病毒的感染及垂直传播途径的初步探讨.海洋与湖沼,1999,30(3):255-260.

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中国对虾育苗期球形病毒的感染及垂直传播途径的初步探讨
汝少国¹, 姜明², 李永祺¹, 马英杰¹
1.青岛海洋大学海洋生命学院青岛266003;2.青岛海洋大学测试中心青岛266003

摘要:

1994–1995年在山东文登市高岛盐厂对虾育苗池和养虾池,随机和定点采集中国对虾的卵、无节幼体、渔状幼体、糠虾幼体、仔虾及幼虾、成虾样品,利用透射电子显微镜对其进行病毒的检测。结果表明,卵细胞中含有大量的卵黄,没有明显的细胞分化,卵细胞质中有球形病毒感染;无节幼体的肝胰腺和上皮细胞的分化程度较低,有大量球形病毒分布;糠虾幼体已有明显的肝胰腺和上皮细胞的分化,肝胰腺上皮细胞的胞质中发现有球形病毒包涵体和球形病毒存在;仔虾期肝胰腺上皮细胞质内有球形病毒感染;幼、成虾球形病毒感染的主要组织为肝胰腺、中肠、肌肉等组织。球形病毒的直径约为70-100nm,具完整的核衣壳,核衣壳外有不光滑的包膜,形成包涵体。育苗期和养成期对虾均有球形病毒感染,推测中国对虾球形病毒有可能经卵传播给子代。

关键词: 中国对虾球形病毒垂直传播透射电子显微镜

DOI：

分类号:

基金项目:国家攀登计划B资助项目，PDB-6-7-1号

附件

STUDIES ON THE VERTICAL TRANSMISSION OF SPHEROID VIRUS IN PENAEUS CHINENSIS

RU Shao-guo¹, JIANG Ming², LI Yong-qi¹, MA Ying-jie¹

1.College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003;2.Center of Analysis, Ocean University of Qingdao, Qingdao, 266003

Abstract:

In 1994 and 1995, eggs and larvae of Penaeus chinensis were collected from prawn seedling ponds in Gaodao, Wendeng, Shandong Province. To determine the virus infection, samples of different stages of P. chinensis larvae and eggs were observed by a transmission electron microscope. The results show that there is a lot of yolk in the egg, but no remarked cell differentiation, and there is spheroid virus infection in the cytoplasm of eggs. There is slight differentiation in the hepato-pancreatic and epidermal cells, with a lot of spheroid virus in them. There are distinguishable differentiation in the hepato-pancreatic cells and a lot of spheroid virus and its inclusion body in the epidermal cell cytoplasm. Spheroid virus infection occurs in the hepato-pancreatic cells of post larvae. In the juvenile and adult prawns, the spheroid virus mainly infects the hepato-pancreatic tissue, mid-gut muscles etc. The spheroid virus encysts are scattered in the cytoplasm with a diameter of about 70-100nm. The virus has a complete capsid, with a layer of rough membrane outside the capsid. It has also an inclusion body. That virus was observed in eggs strongly suggests the possibility of vertical transmission by eggs.

Key words: Penaeus chinensis, Spheroid virus, Vertical transmission, Transmission electron microscope