| 摘要: |
| 根据荧光测量方法的原理，采用双光路、双通道及单片微机控制、数据自动采集的技术，于1997年研制成功用于海洋现场探测的单波段水中油荧光计。应用该水中油荧光计进行了海水中微量抽份的测量实验研究。测量实验结果表明了，与其相同的测量实验条件下，进行同步取样与用实验室Rf-540荧光分光光度计的测量结果相比较具有良好的相关性，相关系数r?0.995。在没有荧光污染（如黄色物质、无机物和浮游生物物质）的条件下，该水中油荧光计对水中油价的探测浓度范围为：1×10-9g/ml－1×10-5g/ml。 |
| 关键词: 海水油 荧光测量方法 单波段 水中油荧光计 |
| DOI： |
| 分类号: |
| 基金项目:山东省专项资助项目，鲁科计（93）第28号;国家海洋局资助项目，国海科计（93）第218号 |
附件 |
|
| EXPERIMENTAL STUDY ON FLUORIMETRY FOR MEASURING OIL IN SEAWATER |
|
XIA Da-ying, WANG Zhen-xian, ZHANG Shi-kui, LI Bin, XIN Hai-ying, XU Yong-sheng
|
|
First Institute of Oceanography,State Oceanic Administrtion,Qingdao,26003
|
| Abstract: |
| The portable oil measuring instrument, using modern fluorimetry, double channel and single-chip micro-computer control technique, is a new marine optical electronic instrument used for measuring trace-oil in seawater. The instrument can detect fluorescence signals produced by oil in seawater, adopting xanon lamp with low consumption, low repeat frequency and pulse detecting techniques. It works well from sea surface to 25m depth under any light condition.
In September 1997, experimental measurement of the mixture consisting of filtered Qingdao seawater and oil was performed in a laboratory tank, using the oil in-situ fluorometer (OISF). Various oil concentrations were made by addition of known amounts of oil insolution to a known volume of water, the fluorescence was measured for each concentration and the calibration curves were acquired. The experimental results show that under the same experimental condition, correlation between the OISF data and laboratory fluorometer Rf-540 data is very well with r?0.995. Assuming no fluorescence contaminants (e.g. yellow, inorganic and planktonic substances) its measuring range for oil in the water is from 1×10-9g/ml to 1×10-5g/ml.
In practical marine measurements, the influence of natural fluorescence substance on measuremental results cannot be eliminated fully, due to the limit of excitation and emission filters of the oil in-situ fluorometer. Therefore, the effect of fluorescence contaminants must be taken into account when in-water oil is measured by the fluorometer. While in-situ measurement is performed, surface water samples should be collected and analyzed by the fluorometer and GC/MC instruments. Finally, the acquired sample concentration and analytic results of the yellow substance, plankton et al. should be used for correcting and validating calibration curves of the fluorometer in laboratory. |
| Key words: Oil in the Seawater, Fluorimetry, Single channel, Underwater oil fluorometer |
