| 摘要: |
| 于1990年5月-1991年5月，运用透射电镜观察绿色杜氏藻，对该藻被膜泡囊的形态结构及其在细胞内的分布和形成过程进行研究。观察表明，绿色杜氏藻细胞的高尔基体和细胞膜附近有许多大小不等的被膜泡囊；这些被膜泡囊由泡囊及其表面的网状结构构成，其直径在45-120nm之间；在细胞膜附近的被膜泡囊直径较大；在高尔基体和细胞膜上还观察到被膜泡囊的形成过程。结果提示：在处于指数生长期的绿色杜氏藻细胞中，被膜泡囊起着细胞内大分子物质运输的作用。 |
| 关键词: 绿色杜氏藻 被膜泡囊 藻类 电镜 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39070448号;中法合作研究项目 |
附件 |
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| ULTRASTRUCTURAL STUDY OF COATED VESICLES IN DUNALIELLA VIRIDIS TEOD |
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HUANG Xiao-hang1, ZHANG Xiao-qing1, WU Chao-yuan1, Alfsen Annette2
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1.Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071;2.laboraloire d'Erat's lies Moleculaires, C. N. R S. France, Paris, 75006
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| Abstract: |
| Ultrastructural study was conducted on coated vesicles, their distribution and formation in Dunaliella viridis Teod in the algal physiology laboratory of the Institute of Oceanology from May 1990 to May 1991. Cells were cultivated under the light of 5000hx (12h:12h) and at the temperature of 21±1°C until an exponential growth phase was reached. They were collected and fixed with 3% glutaraldehyde and 1% O5O4, in 0.2mol/L phosphate buffer (pH=7.4), dehydrated through ethanol series and embedded in Epon resin afterwards. Sample sections were poststained with uranyl acetate and lead citrate and observed in a Hitachi 500 transmission electron microscope at 80 kV. The result shows that coated vesicles were spherical and covered with electron dense granules. They were present in large numbers and closely associated with Golgi cistemae and dictyosome (Plate I:1-2). Their sizes ranged between 45 - 72 nm in diameter. Some coated vesicles were budding from or laying near these structures. The similar electron dence granules at the end of the cisternal margins strongly suggest an intrinsic relation between Golgi apparatus and coated vesicles. At the vicinity of plasma membrance, coated vesicles were also observed. They were larger than those associated with Golgi apparatus and their sizes were 65 - 120 nm in diameter. On the plasma membrane, parts of the membrane invaginated towards the inside of the cell and formed coated pits (Plate 1:3). These pits were also surrounded by electron dense granules at the cytosolic side of the membrane (Plate I:4). With the deepening of the invagination, the coated pits were gradually dessociated with plasma membrane and electron dense granules completely covered the surface of the membrane vesicles. In this way, a coated vesicle was formed into the cytoplasm (Plate 1: 5). Preliminary results indicate that the coated vesicles may play a role in intracellular traffic of macro molecules and exchange of material across the plasma membrane in these cells. |
| Key words: Dunaliella viridis Teod, Coated vesicle, Algae, Electron microscope |
