| 摘要: |
| 于1993年3月-1994年4月，在实验室内可控条件下，用坛紫菜自由丝状体为材料，研究温度（13-33°C）、光强[5-40 μmol/(m2*s)]和光周期（8-24h）对其生长发育的影响。结果表明：（1）17-21°C，丝状体生长快速健康；25-29°C，适于孢子囊枝的形成，27°C较佳；丝状体在温度高于29°C时开始死亡，丝状藻丝对高温的耐受力比孢子囊枝差；17-21°C，适于壳孢子的形成。（2）丝状体的生长随着光强的增加和光照时间的延长而加快。（3）实验光强下，光周期≤12h，孢子囊枝的形成量随光照时间的增加而增加；光周期长于12h，孢子囊枝基本不形成。（4）藻丝的色泽随光强的增加由红色趋于黄色，不同温度下，形态和色泽与生长速度和发育程度有关。（5）在实验光强下，壳孢子的形成量与光强关系不大。认为坛紫菜自由丝状体的生长发育与光温条件密切相关，据此，可以调控丝状体的生长发育进程，获得理想的生长发育阶段的材料。 |
| 关键词: 坛紫菜 丝状体 光 温度 生长 发育 |
| DOI： |
| 分类号: |
| 基金项目:中国科学院重点基金资助项目，KS85-302-03号 |
附件 |
|
| RELATIONSHIP BETWEEN LIGHT, TEMPERATURE AND GROWTH, DEVELOPMENT OF CONCHOCELIS OF PORPHYRA HAITANENSIS |
|
Tang Xiaorong, Fei Xiugeng
|
|
Institute of Oceanology , Chinese Academy of Sciences, Qingdao 266071
|
| Abstract: |
| This March 1993 to April 1994 study conducted under controlled conditions of temperature (13 - 33°C), light intensity [5 - 40μmol/(m2*s)] and light period (8 - 24h), used free living conchocelis of P. haitanensis from Experimental Marine Biological Laboratory, Chinese Academy of Sciences. Primary conchocelis had 90% filament Conchocelis was blended to 0.5-1 mm per filament and cultured in nutrients supplemented seawater, under the above conditions and shaken regularly. The state of growth and development was examined weekly. Seawater was precipitated in the dark for more than one month. Improved PES (0.0079 mmol/L KI was added) was added to boiled seawater. Standards of growth were conchocelis state observed under microscope (experiments in different light intensity and temperature) and wet weight of conchocelis (experiments in different light period). Standard of development was sporangial branchlets area percentage of total conchocelis estimated microscopically.
(5 - 10) ×10-6 g/ml GeO2 was used to inhibit diatoms and not added when culture liquid was changed next. Because of the limitation of experimental conditions, the upper limit of light intensity was 40 μmol/(m2*s) and not high enough to match that of nature. The results only reflected qualitatively the relationship between light intensity and growth and development. Results were as follows: (1) At 17 - 21°C, conchocelis grew fast and healthily; 25 - 29°C was suitable for formation of sporangial branchlets; 27°C was optimum. The conchocelis began to die at temperature higher than 29°C. Filaments could not tolerate so high temperature as sporangial branchlets. Conchospore formed at 17-21°C. (2) Growth of conchcelis increased with increase of light intensity and light period. (3) In experimental light intensity, sporangial branchlets increased in number with increase of light period not more than 12h and could not form when the light period was more than 12h. (4) Conchocelis turned yellowish from reddish with increase of light intensity; and the conchocelis morpha and color related to growth speed and degree of development at different temperatures. At the temperature range of development, the higher the temperature, the deeper the conchocelis color and the nearer to 1 was the cell's length-to-width ratio. (5) Quantity
of conchospore formation had little relation to light intensity at its experimental range. Conclusion: The growth and development of free living conchocelis of P. haitanensis are closely related to light and temperature, and so, can be controlled artificially to obtain ideal materials at different developmental stages. |
| Key words: Porphyra haitanensis, Conchocelis, Light, Temperature, Growth, Development |
