| 摘要: |
| 于1993年9月-1994年8月，对采自安徽省宁国县的长江华溪蟹，利用电镜技术，并结合组织学和组织化学方法，研究其输精管的超微结构与精荚的形成过程，目的在于为深入研究华溪蟹的进化、受精生物学以及种质保存等提供必要的基础理论依据。结果表明：精子分别进入输精细管，被上皮细胞分泌物所包围；随着精子在管中的后移，分泌物不断增多增厚，最后将精子包围成团进而形成圆柱状精荚；精荚由精子群、精荚基质和精荚壁三部分组成；精荚壁单层，较薄；输精管上皮细胞单层，多核，一般2-5枚，核内具数个核仁；胞质内充满大量粗面内质网和高尔基复合体及其睹泡与小泡，泡内含絮状或颗粒状物质；游离核糖体随处可见；线粒体相对较少；输精细管形成精荚，贮精囊分泌精液并贮存精荚。 |
| 关键词: 长江华溪蟹 输精细管 精荚 |
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| ULTRASRUCTURE OF VAS DEFERENS AND FORMATIGN OF SPERMATOPHORE OF FRESHWATER CRAB, SINOPOTAMONYANGTSEKIENSE (CRUSTACEA, DECAPODA) |
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Wang Lan, Du Nanshan, Lai Wei
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Department of Biology,East China Normal University, Shanghai 200062
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| Abstract: |
| This September 1993 to August 1994' study (by electronmicroscopy, histological, and biochemical methods) on the ultrastructure of the vas deferens and the formation of spermatophoers of the freshwater crab Sinopotamon yangtsekiense collected from Anhui Province showed that sperms enter the anterior vas deferens individually and were distributed randomly in a flocculent substance secreted by the cells of the anterior vas deferens. As the epithelium secretion in the anterior vas deferens increased and became thicker, the sperms moving in the anterior vas deferens aggregate in masses that form the cylindrical spermatophores entering into the seminal vesicle finally. The spermatophore consists of sperm mass, spermatophore matrix and the thin single layer, medium electron density spermatophore wall composed of a lot of high density fibrils matrix and flocculent and layered circular vesicles. The single-layer epithelium of the vas deferens is divided into highly columnar, columnar and flat types. The epithelial cells are multinucleate and generally with 1 - 5 nuclei each. These are a few nucleolis in each nucleus. The cytoplasm contains vast arrays of rough endoplasmic reticulum, Golgi complexes and their cisternae or vesicles containing flocculent and granulose material. The anterior vas deferens is the site for spermatophore formation and the seminal vesicle is the storage site of spermatophores. |
| Key words: Sinopotamon yangtsekiense, Anterior vas deferens, Spermatophore |
