引用本文:	王永华,焦念志.顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究.海洋与湖沼,1996,27(1):46-50.

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顶空气相色谱法测定海水二甲基硫和浮游植物细胞二甲基硫丙酸的研究
王永华¹, 焦念志²
1.北京大学城市与环境学系北京100871;2.中国科学院海洋研究所青岛266071

摘要:

建立了顶空GC/FPD测定海水中二甲基硫（DMS）和浮游植物细胞中二甲基硫丙酸（DMSP）的方法，并研究盐度、温度、气液相比DMS诸因素对DMS项空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6％，平均回收率为106％，最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS，在50°C下作用时间不少于6h，峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾的实际样品分析结果表明，本法可用于一般海洋样品的测定。

关键词: 二甲基硫二甲基硫丙酸顶空气相色谱海水浮游植物细胞

DOI：

分类号:

基金项目:国家自然科学基金!49476298;中国科学院院长基金

附件

DETERMINATION OF DIMETHYLSULFIDE IN SEAWATER AND DIMETHYLSULPHONIOPROPIONATE IN PHYTOPLANKTON CELLS BY HEADSPACE GC/FPD METHOD

Wang Yonghua¹, Jiao Nianzhi²

1.Department of Geogrophy, Beijing University, Beijing 100871;2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071

Abstract:

This paper presents a modified Headspace GC/FPD method for determination of dimethylsulfide (DMS) in seawater and dimethylsulphoniopropionate (DMSP) in phytoplankton cells. The effects of temperature, gas/liquid phase ratio, and salt concentration on headspace sensitivity were investigated. The optimum condition of these three factors are at 40°C, 0.5% and 20% respectively. The method ’s detection limit is 20 ng/L. The relative standard deviation is less than 6%, the average recovery rate is 106%; relative error is less than 10%. The double logarithm linear correlation cient between peak height and DMS concentrations is better than 0.99. The completion time of DMS release from cell DMSP under alkaline condition (10 mol/L NaOH) is at least 6 hours. This procedure can be used for the measurement of DMS and DMSP in a variety of natural samples.

Key words: Dimethylsulfide (DMS), Dimethylsulphoniopropionate (DMSP), Headspace, Gas chromatograph, Seawater, Phytoplankton cell