| 摘要: |
| 于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。 |
| 关键词: 海带 雌配子体 原生质体 鲍酶 纤维素酶 |
| DOI： |
| 分类号: |
| 基金项目:国家科委资助;山东省科委合同攻关项目 |
附件 |
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| ISOLATION AND CULTURE OF FEMALE HAPLOID PROTOPLASTS FROM LAMINARIA JAPONICA |
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Qin Song, Wang Xihua, Tong Shun, Zeng Chengkui (C. K. Tseng)
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Institute of Oceanology, Academia Sinica, Qingdao 266071
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| Abstract: |
| Female haploid protoplasts were isolated in January to April, 1993 from female gametophytes of Laminaria japonica using abalone enzymes extracted from the digestive organs of the abalone Haliotis discus hannai. Single cells and protoplasts could be isolated effectively from filamentous gametophytes after incubation with 1% (W / V) abalone enzymes and 2%(W / V) cellulase (Onozyka R-10) at 25°C. Microscopy and fluorescent microscopy revealed that viable female haploid protoplasts were isolated, and that cell walls were regenerated after 2 days. |
| Key words: Laaninaria japonica, Female gametophyte, Protoplast, Abalone enzymes, Cellulase |
