| 摘要: |
| 于1989年1月—1989年8月,用连续培养和半连续培养方法进行了伪矮海链藻碳水化合物、蛋白质和生化组成比率的日变化同光强、营养盐关系的研究。结果表明,碳水化合物、碳水化合物同叶绿素a和蛋白质的比值均呈现光照期升高而黑暗期降低的昼夜节律;蛋白质在光照期和黑暗期均有合成,但是,光照期的增长速率大于黑暗期的;蛋白质同叶绿素a的比值无明显的日变化。各指标的日变化幅度均随光强的增强和营养盐供应速率的增加而升高,因此,光和营养盐是影响各指标日变化的重要因素。 |
| 关键词: 伪矮海链藻 昼夜节律 碳水化合物 蛋白质 生化组成比 |
| DOI： |
| 分类号: |
| 基金项目:美国海军研究基金资助项目，N14-87-K-0311-1 |
附件 |
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| EFFECTS OF LIGHT AND NUTRIENT ON THE DIEL VARIATION OF THALASSIOSIRA PSEUDONANA Ⅱ. CARBOHYDRATE, PROTEIN SAND RATIO OF BIOCHEMICAL COMPONENTS |
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Yang Xiaolong, Zhu Mingyuan
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First Institute of Oceanography, SOA, Qingdao 266003
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| Abstract: |
| During the period from January 1989 to Augusts 1989 continuous and semi-continuous culture methods were used to study the relationships between light and nutrient and the diel variation of carbohydrate, protein and ratios of biochemical components in T. pseudonana. The results indicate that the diel rhythm of all the parameters studied coincided with the light and dark cycles. Carbohydrate, CHO/Chl a and CHO/PRO exhibited a diel rhythm of increasing rate during the light period and decreasing during the dark period. Protein was continuously synthesized throughout the day and night, whereas, its increasing rate was greater in the light period than that in the dark period. The cellular content of protein and PRO/Chl a ratio were relatively constant during the day.
At low light intensity, the specific increase rates of all parameters were relatively low; with increase of light intensity, μCHO, μPRO, μCHO/PRO increased exponentially, but μPRO/Chl a was constant at all light intensities. At highest light intensity, with the exception of μCHO, μCHO/Chl a, μCHO/PRO did not show any rate decrease. μCHO, μCHO/Chl a, μCHO/PRO increased linearly and μPRO increased hyperbolicly with the increase of growth rate. Nutrients had no effect on the diel variation of PRO/Chl a.
It can be concluded from our experiment that light and nutrient affected the changing amplitude of diel variation in the parameters studied except PRO/Chl a. Thus light and nutrient must be considered when we study the diel variation of biochemical components and use these parameters as the index of physiological state in phytoplankton. The mechanism and significance of the diel variation of these parameters are also discussed in this paper. |
| Key words: Thalassiosira pseudonana, Diel rhythm, Carbohydrate, Protein, Ratios of biochemical components |
