首页 | 期刊介绍 | 编委会 | 道德声明 | 投稿指南 | 常用下载 | 过刊浏览 | 期刊订阅 | In English
引用本文:
【打印本页】   【下载PDF全文】   查看/发表评论  下载PDF阅读器  关闭
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 126次   下载 81  
分享到: 微信 更多
细胞穿膜肽转导大型海藻及对其光合作用的影响*
韦一凡1,2, 郇丽1,2, 牛建峰1, 何林文1, 黄爱优1, 张宝玉1, 林阿朋1, 王广策1
1.中国科学院海洋研究所 青岛;2.中国科学院大学 北京
摘要:
提要 细胞穿膜肽能够介导外源物质进入细胞,是一种简单高效的分子运输手段。传统的大型海藻外源物质转导方法较为复杂,并且效率偏低。目前尚没有将细胞穿膜肽应用于大型海藻的报道。本实验利用细胞穿膜肽九聚精氨酸(R9)对浒苔、紫菜和海带这三种常见的大型海藻进行转入,观察其转导效果,并测定转导后藻体光合作用效率的变化。实验结果表明,细胞穿膜肽R9能够成功的转入三种大型海藻,转入的效率与藻体细胞壁结构有一定联系;转导后藻体的Fv/Fm、ETR(I)和ETR(II)没有明显变化,说明R9对大型海藻的光合作用没有负面影响。本实验建立了一种新型的大型海藻外源物质转导方法,为今后的大型海藻育种,分子转化以及外源物质转入机制等研究奠定了基础。
关键词:  细胞穿膜肽  九聚精氨酸  大型海藻  光合作用效率
DOI:
分类号:
基金项目:
TRANSLOCATIONS OF CELL PENETRATING PEPTIDES ON MARINE MACROALGAE AND ITS EFFECTS ON THE PHOTOSYNTHESIS OF MACROALGAE
Wei Yifan1,2, HUAN LI1,2, NIU JIANFENG1, HE LINWEN1, HUANG AIYOU1, ZHUANG BAOYU1, LIN APENG1, WANG GUANGCE1
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao;2.University of Chinese Academy of Sciences, Beijing
Abstract:
Cell penetrating peptides (CPPs) are effective molecular tools that can transport exogenous substances into different cells. Traditional methods of importing exogenous substances into macroalgae are more complicated and of low efficiency. Yet, no study had been taken to apply CPPs on macroalgae. In the present study, we utilized cell penetrating peptide nona-arginine (R9) to conduct translocation experiments on three common marine macroalgae Ulva prolifera, Porphyra yezoensis and Laminaria japonica. Then, the photosynthetic efficiency of R9-treated thalli were determined by Dual-PAM. The results showed that R9 can successfully get across the cell barriers of three macroalgae. The efficiency of transductions was closely related to the structures of cell walls. It had no significant change in Fv/Fm, ETR(I) and ETR(II) of macroalgae after being treated with R9, which demonstrated that R9 had no negative impact on the photosynthesis of macroalgae. This study has established a new method of importing exogenous substances into macroalgae, which laid a cornerstone for future investigations of macroalgae breeding, molecular transformation and exogenous substance intake mechanisms.
Key words:  Cell penetrating peptides (CPPs)  nona-arginine  marine macroalgae  photosynthetic efficiency
版权所有 《海洋与湖沼》编辑部 Copyright©2008 All Rights Reserved
主管单位:中国科学技术协会 主办单位:中国海洋湖沼学会 中国科学院海洋研究所
地址:青岛市南海路七号  邮编:266071  电话:0532-82898753  E-mail:ols@qdio.ac.cn
技术支持:北京勤云科技发展有限公司