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细胞穿膜肽转导大型海藻及对其光合作用的影响_*
韦一凡^1,2, 郇丽^1,2, 牛建峰¹, 何林文¹, 黄爱优¹, 张宝玉¹, 林阿朋¹, 王广策¹
1.中国科学院海洋研究所青岛;2.中国科学院大学北京

摘要:

提要细胞穿膜肽能够介导外源物质进入细胞,是一种简单高效的分子运输手段。传统的大型海藻外源物质转导方法较为复杂,并且效率偏低。目前尚没有将细胞穿膜肽应用于大型海藻的报道。本实验利用细胞穿膜肽九聚精氨酸(R9)对浒苔、紫菜和海带这三种常见的大型海藻进行转入,观察其转导效果,并测定转导后藻体光合作用效率的变化。实验结果表明,细胞穿膜肽R9能够成功的转入三种大型海藻,转入的效率与藻体细胞壁结构有一定联系；转导后藻体的Fv/Fm、ETR(I)和ETR(II)没有明显变化,说明R9对大型海藻的光合作用没有负面影响。本实验建立了一种新型的大型海藻外源物质转导方法,为今后的大型海藻育种,分子转化以及外源物质转入机制等研究奠定了基础。

关键词: 细胞穿膜肽九聚精氨酸大型海藻光合作用效率

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附件

TRANSLOCATIONS OF CELL PENETRATING PEPTIDES ON MARINE MACROALGAE AND ITS EFFECTS ON THE PHOTOSYNTHESIS OF MACROALGAE

Wei Yifan^1,2, HUAN LI^1,2, NIU JIANFENG¹, HE LINWEN¹, HUANG AIYOU¹, ZHUANG BAOYU¹, LIN APENG¹, WANG GUANGCE¹

1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao;2.University of Chinese Academy of Sciences, Beijing

Abstract:

Cell penetrating peptides (CPPs) are effective molecular tools that can transport exogenous substances into different cells. Traditional methods of importing exogenous substances into macroalgae are more complicated and of low efficiency. Yet, no study had been taken to apply CPPs on macroalgae. In the present study, we utilized cell penetrating peptide nona-arginine (R9) to conduct translocation experiments on three common marine macroalgae Ulva prolifera, Porphyra yezoensis and Laminaria japonica. Then, the photosynthetic efficiency of R9-treated thalli were determined by Dual-PAM. The results showed that R9 can successfully get across the cell barriers of three macroalgae. The efficiency of transductions was closely related to the structures of cell walls. It had no significant change in Fv/Fm, ETR(I) and ETR(II) of macroalgae after being treated with R9, which demonstrated that R9 had no negative impact on the photosynthesis of macroalgae. This study has established a new method of importing exogenous substances into macroalgae, which laid a cornerstone for future investigations of macroalgae breeding, molecular transformation and exogenous substance intake mechanisms.

Key words: Cell penetrating peptides (CPPs) nona-arginine marine macroalgae photosynthetic efficiency