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基金项目:国家自然科学基金项目 |
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Isolation of Phenanthrene-Degrading Bacteria and Optimization for Degrading Activity of Phenanthere |
Lei Huan1, Huang Yu1, Tian Yun1, Zhen Tianling2
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1.School of Life Sciences, Xiamen University;2.State Key Laboratory of Marine Environmental Science, Xiamen University
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Use subimate-pethod,a PAHs-degrading bacteria was isolated from the surface seawater of Botan oil port im Xiamen,and was denominated as strain H.The strain H was classified to Sphingomonas genus according to its 16Sr DNA sequence.The HPLC result showed that the stain H had degraded 81.9% of phenanthrene(final conc.400mg/L)within five days and the velocity is 5.95mg/(L.h).Using uciform design experimentation to optimize the degradation conditions,the degradation velocity of phenanthrene(498.46726mg/L)was the largest after 47.09892 hours,the density of cells was 0.5(OP600). |
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