| 摘要: |
| X射线分析显微镜是一种快速的非破坏分析方法，可以用来对海藻中特定的元素在组织水平进行定位。本实验采用X射线分析显微镜对1997年2月在日本佐贺县唐津市海岸采集的养殖裙带菜(Undaria pinnatifida Suringar)的孢子叶进行了硫、钾、钙、碘的组织定位研究，对孢子叶的整体进行X射线扫描及特征元素次级荧光的图像分析，并选取孢子叶的边缘部、中部及中心各一点进行定点的X射线荧光光谱测定。Ⅹ射线荧光光谱的结果表明，钾是裙带菜孢子叶的最显著元素，具有最大的X射线荧光强度。定位分析表明，钾在孢子叶的中心部位分布较高，钙与碘则在边缘部分布较高，硫多位于孢子叶的中间部分，在中心部位及边缘部的分布均较低。除了钾、钙、硫以外，其他元素如铁、溴、锌也能够在X射线荧光光谱中给出谱峰。作者得到的X射线特征元素荧光图像首次报道了裙带菜孢子叶中钾、钙、硫等元素在组织水平的定位。应用RGB图像彩色合成技术获得的硫、钙、钾三元素的彩色合成图像也与定点分析结果相一致。 |
| 关键词: 海藻元素、海藻、X射线、裙带菜孢子叶 |
| DOI： |
| 分类号: |
| 基金项目:中国科学院海洋研究所调查研究报告第3726号。 |
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| ELEMENTAL STUDIES ON MARINE ALGAE BY X-RAY ANALYTICAL MICROSCOPE PART Ⅱ. MAPPING OF ELEMENTS IN SPOROPHYLL OF UNDARIA PINNATIFIDA SURINGAR |
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Yan Xiaojun1, Chen Yumin1, Fan Xiao1, Yoshihiro Chuda2, Tadahiro Nagata2
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1.Institute of Oceanology, Chinese Academy of Sciences;2.National Food Research Insititute, Tsukuba, Japan
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| Abstract: |
| Seaweeds can efficiently accumulate almost all the elements in seawater, and serve as good experimental material for elemental analysis. Undaria pinnatifida Suringar, a favorite edible alga, can form distinctive reproductive organs, i.e. sporophylls (Chihara, 1975; Chapman and Chapman, 1973). Sporophyll of Undaria pinnatifida is believed to have great potential for enhancing human health and regarded as potent folklore medicine (Nisizawa, 1989). Some experimental data proved that sporophyll of Undaria pinnatifida is beneficial as cancer-preventive and cardiovascular protective agents (Furusawa and Furusawa, 1985; Oishi, 1993 ). Elements accumulated in the sporophyll can explain the physiological bioactivities of the sporophyll. So it is interesting to know the special elements and their localization in the sporophyll.
X-ray analytical microscopy, a rapid and visual method for analysis of elemental composition, can provide an X-ray transmission image of a sample and fluorescent X-ray analysis of elemental mapping at the same time (Hosokawa, 1995). Using such a non-destructive technique, the distribution of certain elements can be observed from the formed imaged directly if the element is high enough to show a clear image. Here, the authors report the results of their study on the location of major elements such as potassium and calcium in the sporophyll of Undaria pinnatifida. |
| Key words: marine algae,X-ray ,undaria pinnatifida Suringar,sporophyll |
