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一株芘高效降解菌的分离纯化及降解性能的研究
蔡丽希
莆田学院
摘要:
通过富集驯化培养,从红树林污泥中分离到一株以芘作为唯一碳源与能源的高效菌株PY5,经过形态观察、生理生化与16S rDNA 鉴定,PY5属于蜡状芽孢杆菌(Bacillus cereus),同时对该菌株降解芘性能进行了系统研究。结果表明B.cereus对50 mg/L芘的21 d降解率为65.84 %,投加Tween-80、葡萄糖、水杨酸、Fe3+、Mn2+等基质可以不同程度强化降解芘的能力,其中投加葡萄糖+ Fe3+ + Mn2+的混合液对B. cereus降解芘的强化效果最好,21d的芘降解率可达到81.42 %,相对于不加共代谢底物时提升15.58%,表明共代谢体系对高分子量多环芳烃污染降解具有不俗的促进作用。
关键词:    红树林 多环烷烃  降解 共代谢
DOI:
分类号:
基金项目:2013年莆田市科技计划项目( 2013S01(4))
Studies on Isolation and Degradation Characteristics of An Efficient Pyrene-Degrading Stain
Cai Lixi
Department of Basic Medical Science,Putian University
Abstract:
A bacterial strain named PY5 which can utilize pyrene as sole carbon and energy source was separated from the sediment of mangrove. The strain was identified as Bacillus cereus according to the results of physiology, morphology and phylogenetical analyses of 16S rDNA sequence about B.cereus showed high degradation capability with 65.84 % of 50mg/L pyrene within 21days. The adding of Tween-80、glucose、salicylic acid、Fe3+、Mn2+can accelerate the degradation of pyrene by B. cereus. Degradation rate under the glucose + Fe3+ + Mn2+ mixture supplementation was 81.42 % after 21 days, enhanced 15.58% compare to the condition of without cometabolism degradation substrate. The results also showed that the cometabolism degradation is feasible for the degradation of high molecular weight polycyclic aromatic hydrocarbons.
Key words:  Pyrene  Mangrove Polycyclic  aromatic hydrocarbons(PAHs) Degradation  cometabolism
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