| 摘要: |
| 分别从泥蚶血淋巴液和肌肉中提取出凝集素，进行全血微量培养法进行淋巴细胞转化和抑菌实验。结果表明：两种凝集素都具有促进小鼠淋巴细胞转化的免疫调节功能；两种凝集素对供试的金黄色葡萄球菌（Staphylococcus aureus）；甘薯薯瘟病原细菌（Fasarium oxyssporam）； 大肠杆菌（Escherichia coli）；普通变形杆菌（Proteas valgaris）；八叠球菌（ Sarcina lutea）5种细菌和啤酒酵母（Saccharomyces carlsbergensis）；玉米大斑病菌（Helminthos porium turcicum）； 稻瘟病菌（Piricularia oryzae）3种真菌都有不同程度的抑制作用，且对真菌的抑制作用大于对细菌的抑制作用；由二者分别制备的抗血清通过免疫双扩散法并不能与对方发生交叉沉淀反应，说明它们不具有同源性。 |
| 关键词: 泥蚶 凝集素 免疫活性 抑菌作用 |
| DOI： |
| 分类号:Q956 |
| 基金项目:福建省教育厅A类科技项目（JA09213）；泉州师范学院大学生科研基金项目（2009DA023） |
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| The Antibacterial Activity and Immunological Properties of Lectins from Tegillarca granosa Linnaeus |
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kejiaying
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Quanzhou Normal University
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| Abstract: |
| The lymphocyte transformation and antimicrobial experiment are test with two kinds of lectins (TGL-h and TGL-m ) which are extracted from the hemolymph and muscle of Tegillarca granosa. It’s found that two lectins have immune regulating function in lymphocyte transformation; besides, they have different degrees of inhibition for five kinds of bacteria: Staphylococcus aureus; Fasarium oxyssporam; Escherichia coli; Proteas valgaris; Sarcina lutea and three kinds of fungi: Saccharomyces carlsbergensis; Helminthos porium turcicum; Piricularia oryzae. The inhibition of fungi is greater than the inhibition of bacteria. The two antisera does not happen with the other cross-precipitation reaction by double immunodifusion method, indicating that two lectins do not have homology. |
| Key words: Tegillarca granosa Lectins Antibacterial Activity Immunological Properties |