| 摘要: |
| 海洋无脊椎动物在生物医学和农学等领域蕴藏巨大的应用潜力, 从基因层面解析和开发海洋无脊椎动物的生物学价值是未来研究的重要方向。目前模式生物中常见的遗传学操作手段还未广泛应用于海洋无脊椎动物, 因而限制了对海洋无脊椎动物在基因功能、基因表达调控等方面的研究。转染作为一种可高效将外源核酸导入细胞的技术, 是海洋无脊椎动物基因操作的理想工具。首先概述了不同类型的转染方法, 并对其转染的优缺点进行了总结; 随后重点介绍了转染技术在海洋无脊椎动物中的应用进展; 最后总结了在海洋无脊椎动物中建立转染技术的难点。以期为海洋无脊椎动物在转染策略的选择中提供参考, 为海洋无脊椎动物的分子生物学研究提供帮助。 |
| 关键词: 转染 海洋无脊椎动物 物理转染 化学转染 |
| DOI：10.11759/hykx20240417003 |
| 分类号:P735;Q78 |
| 基金项目:国家自然科学基金(32200415) |
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| Current status of the transfection techniques applied in marine invertebrates |
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JI Zhongyu, KANG Yunsi, DI Guotao
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Fang Zongxi Center, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| Marine invertebrates harbor immense potential for applications in many fields, such as biomedicine and agriculture. Unraveling and developing their bio-economical value at the genetic level is a major topic for future research. However, genetic manipulation techniques commonly used in model organisms have not yet been widely applied to them; this limitation restricts research on aspects such as gene function and regulation of expression in them. Transfection, as a highly efficient technique for transfection of cells, has become an ideal tool for the genetic manipulation of marine invertebrates. Here, we provide an overview of the different types of transfection methods and compare their advantages and disadvantages. Then, we focus on the progress of transfection technology and summarize the challenges in establishing transfection techniques in marine invertebrates. This study aims to provide a reference for the selection of transfection strategies and to assist in molecular biology research in marine invertebrates. |
| Key words: transfection marine invertebrates physical transfection chemical transfection |
