引用本文:	刘涛,缪丰锴,娄琦,杜鹏,李正炎.二氧化碳暴露对海水青鳉生存和抗氧化系统的影响[J].海洋科学,2024,48(11):8-.

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二氧化碳暴露对海水青鳉生存和抗氧化系统的影响
刘涛¹, 缪丰锴², 娄琦³, 杜鹏^2,4, 李正炎^2,5
1.中海油研究总院有限责任公司, 北京 100028;2.中国海洋大学环境科学与工程学院, 山东青岛 266100;3.青岛大学环境科学与工程学院, 山东青岛 266071;4.青岛中海昶洋环境科技有限公司, 山东青岛 266061;5.中国海洋大学海洋环境与生态教育部重点实验室, 山东青岛 266100

摘要:

在“双碳”战略背景下,世界沿海国家积极探索海洋碳封存实践,然而迄今人们对碳封存可能引发的二氧化碳泄漏及其对海洋生物尤其是海洋鱼类的毒性效应研究仍然十分匮乏。本文以生态毒理试验模式物种海水青鳉为受试生物,研究二氧化碳暴露对海水青鳉存活和抗氧化系统的影响,并探求其毒性作用机制。结果表明,二氧化碳暴露对海水青鳉48 h和96 h半致死浓度(LC₅₀)分别为20.8 mmol/L和19.5 mmol/L。亚致死暴露条件下(暴露浓度0.87~8.65 mmol/L),海水青鳉体内超氧化物歧化酶(SOD)活性在暴露14 d后呈诱导趋势,但暴露21 d后诱导趋势减缓。过氧化氢酶(CAT)活性随二氧化碳暴露浓度升高受到显著抑制,暴露21 d后的最高无效应浓度(NOEC)和最低效应浓度(LOEC)分别为3.57 mmol/L和6.89 mmol/L。脂质过氧化的生物标志物丙二醛(MDA)含量对二氧化碳暴露响应更敏感,21 d后NOEC和LOEC分别为0.87 mmol/L和1.56 mmol/L。该研究表明二氧化碳暴露可能导致海水青鳉体内自由基累积并引发抗氧化系统损伤。

关键词: 二氧化碳抗氧化系统超氧化物歧化酶过氧化氢酶丙二醛

DOI：10.11759/hykx20240501001

分类号:X55

基金项目:中海石油(中国)有限公司北京研究中心科技项目(CCL2022RCPS0245KCN);国家自然科学基金(U22A20614)

Effects of carbon dioxide on survival and antioxidant system of Oryzias melastigma

LIU Tao¹, MIAO Fengkai², LOU Qi³, DU Peng^2,4, LI Zhengyan^2,5

1.China National Offshore Oil Corporation (CNOOC), Beijing 100028, China;2.School of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;3.College of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;4.Qingdao Zhonghaichangyang Environmental Technology Co Ltd, Qingdao 266061, China;5.Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China

Abstract:

In the context of the “double carbon” strategy, coastal countries around the world are actively exploring marine carbon sequestration practices. However, there is a shortage of knowledge on carbon dioxide leakage caused by carbon sequestration and the subsequent harmful effects on marine life, especially on fish. In this study, marine medaka Oryzias melastigma, an ecotoxicological test model species, was used to investigate the effect of carbon dioxide exposure on its survival and antioxidant system. The results showed that the 48-h and 96-h half-lethal concentrations (LC₅₀s) of CO₂ exposure were 20.82 and 19.47 mmol/L, respectively. Under sublethal exposure conditions (0.87–8.65 mmol/L), the superoxide dismutase (SOD) activity in marine medaka was increased after 14 d of exposure but decreased after 21 d. Catalase (CAT) activity was significantly inhibited with increasing carbon dioxide exposure concentration, and no-observed-effect concentration (NOEC) and lowest-observed-effect concentration (LOEC) values after 21 days of exposure were 3.57 and 6.89 mmol/L, respectively. The content of malondialdehyde (MDA), a biomarker of lipid peroxidation, was more sensitive to CO₂ exposure, with NOEC and LOEC values of 0.87 and 1.56 mmol/L after 21 days, respectively. These results indicate that CO₂ exposure may cause free radical accumulation and trigger antioxidant system damage in marine medaka.

Key words: carbon dioxide antioxidant system superoxide dismutase catalase malondialdehyde