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Cu对云龙石斑鱼幼鱼的生理生态毒性
靳智欣,徐永江,刘新富,梁 友,周鹤庭,崔爱君,刘欣
1.浙江海洋大学国家海洋设施养殖工程技术研究中心,浙江 舟山 316022;2. 中国水产科学研究院黄海水产研究所;3.中国水产科学研究院黄海水产研究所,青岛海洋科学与技术试点国家实验室深蓝渔业工程联合实验室
摘要:
为研究Cu对云龙石斑鱼(Epinephelus moara♀×Epinephelus lanceolatus♂)幼鱼的生态毒性及生理生化指标的影响,分析了Cu2+对幼鱼的安全质量浓度(SC)、组织损伤、酶活力、金属硫蛋白-2(MT-2)基因表达的影响。实验设置对照组以及0.1mg/L、0.2mg/L、0.4mg/L、0.8mg/L、1.2mg/L、2mg/L 等6个Cu2+实验组进行为期5d的毒理实验。结果显示:(1)Cu2+对云龙石斑鱼幼鱼的SC为0.0162mg/L。(2)Cu暴露诱发实验鱼的肝脏组织血窦扩张;鳃组织出现动脉瘤以及顶部棒状等现象。(3)与对照组相比,随Cu2+浓度升高,肝脏组织总超氧化物歧化酶(SOD)活力显著下降;谷草转氨酶(GOT)活力先上升后下降。(4)随Cu处理浓度升高,肝脏、鳃组织MT-2基因表达水平均逐渐上升;而脑组织中MT-2表达水平先上升后下降,但低浓度组较对照组差异不显著。结果表明,高浓度的Cu会造成云龙石斑鱼幼鱼的组织损伤、免疫酶活力降低等一系列不利影响,结果为云龙石斑鱼养殖生产过程中利用铜制剂防控病害的适宜剂量选择提供依据。
关键词:  云龙石斑鱼    安全质量浓度  生理应答  组织损伤
DOI:
分类号:
基金项目:国家重点研发计划项目(2019YFD0900901, 2020YFD0900605); 中国水产科学研究院级基本科研业务费专项课题 (TD47);财政部和农业农村部:国家现代农业产业技术体系(CARS-47)
Physiological and Ecological toxicity of copper on juvenile Yunlong Grouper (Epinephelus moara♀×Epinephelus lanceolatus♂)
Jinzhixin1,2, Xuyongjiang3, Liuxinfu3, Liangyou3, Zhouheting4, Cuiaijun4, Liuxin3
1. National Engineering Research Center For Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China;2.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and , Pilot National Laboratory for Marine Science and Technology (Qingdao) for Deep Blue Fishery Engineering;4.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Laboratory for Marine Fisheries Science and , Pilot National Laboratory
Abstract:
The study was conducted to determine the effects of copper exposure on toxicity, physiology, immunity, histomorphology and gene expression in Yunlong Grouper (Epinephelus moara♀×Epinephelus lanceolatus♂) juveniles. In the present study, the fish were respectively exposed to designed Cu2+ concentrations of 0 (control), 0.1, 0.2, 0.4, 0.8, 1.2 and 2 mg/L for 5 days. The results show that the safe concentration of copper for juvenile Yunlong grouper was 0.0162 mg/L. Copper exposure induced dilatation of sinusoids in the liver, and also induced clubbed tips, and aneurysms in gills. With the increase of Cu2+ concentration, the activity of total superoxide dismutase (SOD) decreased in the liver, and the activity of glutamic-oxaloacetic transaminase (GOT) showed an initial increase followed by a decreasing trend. The expression level of MT-2 mRNA increased in liver and gills induced by copper exposure stress, however, the expression level of MT-2 mRNA increased firstly and then decreased in the brain. In summary, a series of physiological changes such as structure damage in gills, liver and immunity related enzymes activity inhibition in the liver of Yunlong grouper were caused by high Cu exposure concentration, which provides chosen guidance of copper products for disease control in Yunlong grouper aquaculture.
Key words:  Yunlong grouper  Copper  Safe concentration  Physiological stress  Structure damage
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