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海带中岩藻黄素异构体的分离纯化及其体外抗肿瘤活性研究
吴素煌, 王晶, 岳洋, 张全斌
中国科学院海洋研究所
摘要:
海带采用乙醇提取色素,通过柱层析分离纯化其中的岩藻黄素,并最终通过高效制备液相色谱制备了三种岩藻黄素的异构体。通过紫外光谱(UV)、核磁共振氢谱(1H-NMR)以及质谱(MS)对岩藻黄素及其异构体进行了结构表征和鉴定。采用乳腺癌细胞MDA-MB-231模型,对岩藻黄素的体外抗肿瘤活性进行了研究。结果表明,岩藻黄素对乳腺癌细胞生长有显著的抑制作用,其中13-顺和13'-顺岩藻黄素组分对癌细胞的抑制活性最强,IC50值达到23.71 μg/mL。全反式岩藻黄素和9'-顺岩藻黄素两种异构体IC50值分别为45.30 μg/mL和27.09 μg/mL。qPCR试验表明岩藻黄素各异构体可以通过抑制癌细胞NF-κB家族基因表达而抑制乳腺癌细胞生长。
关键词:  岩藻黄素  顺反异构体  乳腺癌  抗肿瘤
DOI:10.11759/hykx20190308001
分类号:
基金项目:山东省重点研发计划项目(2016HYSP0002);中国科学院王宽诚基金会项目
Isolation, identification and the in vitro anti-breast cancer effects of fucoxanthin stereoisomers from Laminaria japonica
WU suhuang, WANG jing, YUE yang, ZHANG quanbin
Institute of Oceanology,Chinese Academy of Sciences
Abstract:
The pigments from Laminaria japonica were extracted with ethyl acetate and the fucoxanthin stereisomers were purified by column chromatography. Finally, three fucoxanthin stereisomers were prepared by preparative high performance liquid chromatography (HPLC). The structures of the fucoxanthin stereisomers were characterized by ultraviolet spectroscopy (UV), mass spectrometry (MS) and nuclear magnetic resonance (NMR). The in vitro antitumor activity of the fucoxanthin stereisomers was studied using MDA-MB-231 breast cancer cell line. The results showed that fucoxanthin isomers possessed great inhibitory effects against the growth of breast cancer cells. The 13-cis and 13'-cis fucoxanthin mixtures showed the highest inhibitory activity, and the IC50 value was 23.71 μg/mL. In addition, the IC50 values of the all trans-fucoxanthin and the 9'-cis-fucoxanthin were 45.30 μg/mL and 27.09 μg/mL, respectively. Further, the qPCR experiment suggested that the fucoxanthin stereisomers may inhibit the growth of breast cancer cells by down-regulating the expression of NF-κB related genes.
Key words:  fucoxanthin  stereoisomer  breast cancer  antitumor activity
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